Prevent excessive backpressure or replace gasket When particle size of stationary phase is 5 to 20 µm, use frit 2 µm. *Note: When the particle size of stationary phase is 3 to 4 µm, use frit 0.5 µm. Keep waste line above surface vaste, with proper slope Tighten valve or replace it if it is faulty Tighten check valve (do not overtighten) or replace check valveĬheck diaphragms, replace if leaky and/or check for fitting damage, replace If the fitting is damaged, replace it.ĭisassemble fitting and clean it. If the fitting or ferrule is damaged, replace it with new one. distored ferrule, or particles on the sealing surface). If a fitting leak does not stop when the fitting is tightened a little, take the fitting apart and inspect for damage (e.g.
Be aware, however, that overtightened metal compression fittings can leak and plasrtic fingertight fittings can wear out. Leaks are usually stopped by tightening or replacing a fitting. Pressure cycling is normal due to viscosity change See section Low pressure Pressure cycling Possible caseĭegas solvent and/or bleed air from the pumpĭegas solvent and/or change degassing methods (e.g. See sections No pressure reading, no flow and No pressure reading, flow is normal Pressure dropping, but not to zero precipitation of sample or buffer in column.clogged or damaged pre-column filter, guard column or frit.If the pressure is normal, there is a problem with the column: If high back pressure still appears, then the blockage is in the system: Remove column from the system and turn on pump. You should find out, what caused high back pressure - column or system? We recommend following procedure: Improper mobile phase, precipitated buffer Now pressure reading, flow is normal Possible causeīackflush column if it is permitted, replace frit according to the manufacturer's instructions and warratny conditions or replace column Replenish reservoir, replace inlet frit if it is blocked Verify proper settings, repaire or replace controllerĭegas mobile phase, bleed air from pump and prime pump » Chromatography » Hints and tips » HPLC troubleshooting Chromatography / Hints and tips / HPLC troubleshooting HPLC troubleshooting Abnormal pressure No pressure reading, no flow Possible cause
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